Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata
Language English Country United States Media print
Document type Journal Article
PubMed
8181783
DOI
10.1007/bf02814521
Knihovny.cz E-resources
- MeSH
- Actinomycetales enzymology MeSH
- Bacterial Proteins isolation & purification MeSH
- Chemical Precipitation MeSH
- Chromatography, Affinity MeSH
- DNA-Directed RNA Polymerases isolation & purification MeSH
- Chromatography, Gel MeSH
- Molecular Weight MeSH
- Spores, Bacterial MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Bacterial Proteins MeSH
- DNA-Directed RNA Polymerases MeSH
DNA-Dependent RNA polymerase (EC 2.7.7.6) was isolated from Thermomonospora curvata. The purification steps included precipitation with Polymin P, elution of the precipitate with 0.3 mol/L KCl, precipitation with ammonium sulfate, affinity chromatography on heparin-agarose and molecular filtration on Biogel A 1.5 m.
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