Characterization of the alpha-amylase-encoding gene from Thermomonospora curvata

. 1992 Mar 01 ; 112 (1) : 77-83.

Jazyk angličtina Země Nizozemsko Médium print

Typ dokumentu srovnávací studie, časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/pmid01551601
Odkazy

PubMed 1551601
DOI 10.1016/0378-1119(92)90305-9
PII: 0378-1119(92)90305-9
Knihovny.cz E-zdroje

The nucleotide sequence of a 3007-bp DNA fragment from Thermomonospora curvata CCM3352 containing the coding and regulatory region of the alpha-amylase-encoding gene (tam) was determined. Primer extension mapping was used to determine the 5' end of the transcript, and it was demonstrated that the gene is transcribed from a unique promoter which is also functional in Streptomyces lividans TK24. Transcription of tam in T. curvata was induced by maltose, even in the presence of glucose. In S. lividans, tam was expressed constitutively. The deduced amino acid sequence reveals a considerable similarity with alpha-amylases from streptomycetes.

Citace poskytuje Crossref.org

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GENBANK
M76680, M76682, M79307, M79308, M79309, M79310, X13839, X59159, X60732, X60733

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