Characterization of the alpha-amylase-encoding gene from Thermomonospora curvata
Language English Country Netherlands Media print
Document type Comparative Study, Journal Article
PubMed
1551601
DOI
10.1016/0378-1119(92)90305-9
PII: 0378-1119(92)90305-9
Knihovny.cz E-resources
- MeSH
- Actinomycetales enzymology genetics MeSH
- alpha-Amylases chemistry genetics MeSH
- Glucose pharmacology MeSH
- Cloning, Molecular MeSH
- Maltose pharmacology MeSH
- Molecular Sequence Data MeSH
- Promoter Regions, Genetic genetics MeSH
- Gene Expression Regulation, Bacterial drug effects MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Streptomyces enzymology genetics MeSH
- Temperature MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- alpha-Amylases MeSH
- Glucose MeSH
- Maltose MeSH
The nucleotide sequence of a 3007-bp DNA fragment from Thermomonospora curvata CCM3352 containing the coding and regulatory region of the alpha-amylase-encoding gene (tam) was determined. Primer extension mapping was used to determine the 5' end of the transcript, and it was demonstrated that the gene is transcribed from a unique promoter which is also functional in Streptomyces lividans TK24. Transcription of tam in T. curvata was induced by maltose, even in the presence of glucose. In S. lividans, tam was expressed constitutively. The deduced amino acid sequence reveals a considerable similarity with alpha-amylases from streptomycetes.
References provided by Crossref.org
WD-repeat protein encoding genes among prokaryotes of the Streptomyces genus
Isolation of DNA-dependent RNA polymerase from the thermophilic actinomycete Thermomonospora curvata
GENBANK
M76680, M76682, M79307, M79308, M79309, M79310, X13839, X59159, X60732, X60733
