Elektronovĕ mikroskopický průkaz viru epidemické diarrhoey prasat v Ceské republice
[Detection of porcine epidemic diarrhea virus using electron microscopy in the Czech Republic]
Language Czech Country Czech Republic Media print
Document type English Abstract, Journal Article
PubMed
8394039
- MeSH
- Coronaviridae classification MeSH
- Microscopy, Electron MeSH
- Microscopy, Immunoelectron MeSH
- Coronaviridae Infections microbiology veterinary MeSH
- Swine Diseases microbiology MeSH
- Swine MeSH
- Diarrhea microbiology veterinary MeSH
- Intestines microbiology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
Coronavirus-induced porcine epidemic diarrhoea (PED) was diagnosed in two swine herds. The causal agent was demonstrated in intestinal contents by electron microscopy and identified by immunoelectron microscopy using specific immune serum to the reference strain PED-CV77. Experimental transmission to hysterectomy-derived, colostrum-deprived piglets with an intestinal contents filtrate was successful. The virus was demonstrable by electron microscopy in the intestinal contents between 12th hour and 4th day, and in small intestinal epithelial cells 18 hours after infection. Scanning electron microscopy revealed shortening and fusion of villi of small intestinal mucosa.
An ELISA optimized for porcine epidemic diarrhoea virus detection in faeces
