Peroxidase-activated carcinogenic azo dye Sudan I (Solvent Yellow 14) binds to guanosine in transfer ribonucleic acid
Jazyk angličtina Země Slovensko Médium print
Typ dokumentu časopisecké články
PubMed
8529865
Knihovny.cz E-zdroje
- MeSH
- barvicí látky metabolismus MeSH
- guanosin metabolismus MeSH
- karcinogeny metabolismus MeSH
- krysa rodu Rattus MeSH
- kyselina 5'-guanylová metabolismus MeSH
- naftoly metabolismus MeSH
- nukleotidy metabolismus MeSH
- peroxidasa metabolismus MeSH
- polyribonukleotidy metabolismus MeSH
- RNA transferová metabolismus MeSH
- techniky in vitro MeSH
- vazebná místa MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 1-phenylazo-2-naphthol MeSH Prohlížeč
- barvicí látky MeSH
- guanosin MeSH
- karcinogeny MeSH
- kyselina 5'-guanylová MeSH
- naftoly MeSH
- nukleotidy MeSH
- peroxidasa MeSH
- polyribonukleotidy MeSH
- RNA transferová MeSH
Peroxidase in the presence of hydrogen peroxide catalyzes in vitro the activation of the carcinogenic azo dye Sudan I (1-phenylazo-2-hydroxynaphthalen) to tRNA-, homopolyribonucleotide- and 5'-monophosphate nucleoside-bound products. tRNA, poly G and guanosine 5'-monophosphate modified by activated Sudan I become colored and have an absorption maximum of approx. 480 nm. Cochromatographic analysis of adducts obtained by a reaction with tRNA and guanosine 5'-monophosphate on a thin layer of cellulose showed that the major Sudan I-tRNA adduct was formed by a reaction of activated Sudan I with guanosine in tRNA. The radical mechanism of the binding of the Sudan I molecule, containing the whole azo aromatic system, to nucleic acids is discussed.
