Multifactorial induction of gene expression and nuclear localization of mouse interleukin 1 alpha
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8818542
DOI
10.1006/cyto.1996.0062
PII: S1043-4666(96)90062-9
Knihovny.cz E-zdroje
- MeSH
- buněčná diferenciace MeSH
- buněčné jádro chemie MeSH
- hnědá tuková tkáň chemie MeSH
- interleukin-1 chemie metabolismus MeSH
- kultivované buňky MeSH
- lipopolysacharidy farmakologie MeSH
- messenger RNA metabolismus MeSH
- molekulová hmotnost MeSH
- myši MeSH
- noradrenalin farmakologie MeSH
- northern blotting MeSH
- receptory interleukinu-1 chemie MeSH
- regulace genové exprese MeSH
- subcelulární frakce chemie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- interleukin-1 MeSH
- lipopolysacharidy MeSH
- messenger RNA MeSH
- noradrenalin MeSH
- receptory interleukinu-1 MeSH
We have studied mouse brown adipose tissue (BAT) which was shown to contain high levels of mRNA for IL-1 alpha and IL-1 receptor. We found high contents of both the 31 kDa precursor and the 18 kDa mature form of IL-1 alpha proteins in BAT when compared with brain, lymph nodes and spleen. Both forms of IL-1 alpha were also present in primary cultures of BAT. The IL-1 alpha subcellular localization revealed the predominant nuclear association of both precursor and mature form of IL-1 alpha which accounted for 48% of total cellular IL-1 alpha in BAT and a similar subcellular distribution was found in spleen. The specific content of IL-1 alpha found in nuclei purified from BAT or from cultured brown adipocytes was 6-47-fold higher than in other cellular fractions. Nuclear IL-1 alpha was increased by addition of 10% fetal calf serum to cultured adipocytes previously depleted of the serum. The expression of the IL-1A gene in cultured brown adipocytes was increased 6-8-fold by IL-1 beta, TNF-alpha, LPS and by noradrenaline. The stimulation with all four agents, resulted in a rapid burst of IL-1 alpha mRNA level after 15-30 min. The highest stimulation was found in differentiated, preconfluent adipocytes cultured for 6 days. The results suggest an important regulatory role of IL-1 alpha in brown adipocytes. The rapid responses of IL-1A gene expression to multifactorial stimulation and pronounced nuclear localization of IL-1 alpha proteins further support the existence of a hypothetical nuclear function of IL-1 alpha.
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