Determination of furosemide in plasma and urine by gas chromatography/mass spectrometry
Language English Country Germany Media print
Document type Clinical Trial, Journal Article
PubMed
8901149
Knihovny.cz E-resources
- MeSH
- Bumetanide chemistry MeSH
- Diuretics analysis pharmacokinetics MeSH
- Furosemide analysis pharmacokinetics MeSH
- Calibration MeSH
- Humans MeSH
- Kidney Diseases blood urine MeSH
- Gas Chromatography-Mass Spectrometry MeSH
- Half-Life MeSH
- Quality Control MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Clinical Trial MeSH
- Names of Substances
- Bumetanide MeSH
- Diuretics MeSH
- Furosemide MeSH
A new method for the determination of furosemide (CAS 54-31-9) in plasma and urine by gas chromatography/mass spectrometry (GC/MS) has been developed. After acidification the samples were extracted by ethyl acetate and methylated by methyl iodide. The chromatography was carried out on a fused-silica capillary column with SE-54 stationary phase. Detection was performed by selected ion monitoring (ions 81 and 372 were monitored for furosemide and ions 363 and 406 for internal standard bumetanide). Limit of quantitation was 10 ng/ml for plasma and 40 ng/ml for urine and the calibration curves were linear up to 100,000 ng/ml.
