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Towards the two-dimensional imaging of spontaneous ultra-weak photon emission from microbial, plant and animal cells
A. Prasad, P. Pospíšil,
Language English Country England, Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
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PubMed
23386970
DOI
10.1038/srep01211
Knihovny.cz E-resources
- MeSH
- Arabidopsis physiology MeSH
- Time Factors MeSH
- Photons * MeSH
- Humans MeSH
- Oxidation-Reduction MeSH
- Hand physiology MeSH
- Saccharomyces cerevisiae physiology MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Two-dimensional imaging of spontaneous ultra-weak photon emission was measured in the yeast cells, Arabidopsis plant and the human hand using highly sensitive charge coupled device (CCD) camera. For the first time, the detail analysis of measuring parameters such as accumulation time and binning is provided with the aim to achieve two-dimensional images of spontaneous ultra-weak photon emission of good quality. We present data showing that using a hardware binning with binning factor 4 × 4, the accumulation time decreases in the following order: yeast cells (30 min) > the human hand (20 min) > Arabidopsis plant (10 min). Analysis of measuring parameters provides a detailed description of standard condition to be used for two-dimensional spontaneous ultra-weak photon imaging in microbes, plants and animals. Thus, CCD imaging can be employed as a unique tool to examine the oxidative state of the living organism with the application in microbiological, plant and medical research.
References provided by Crossref.org
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