Capillary electrophoresis of cytokinins and cytokinin ribosides
Language English Country Netherlands Media print
Document type Journal Article
PubMed
9099002
DOI
10.1016/s0021-9673(96)00927-2
PII: S0021-9673(96)00927-2
Knihovny.cz E-resources
- MeSH
- Cytokinins analysis MeSH
- Electrophoresis, Capillary methods MeSH
- Ribonucleosides analysis MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Cytokinins MeSH
- Ribonucleosides MeSH
A CE separation of cytokinins and cytokinin ribosides and some other purine and pyrimidine bases has been developed. Two electrolyte systems have been tested: 150 mM phosphoric acid, pH 1.8 and 50 mM sodium dodecylsulphate + 20 mM borate, pH 9.2. The migration times were measured and the effects of the solute structures were discussed. Preliminary experiments with plant extracts have been performed to identify the cytokinins and their ribosides. Both the systems can be used, but 150 mM phosphoric acid is better suited for identification of cytokinins in plant extracts, as the electropherograms are subject to fewer interferences.
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