In vitro and in vivo transcription from a computer predicted promoter
Language English Country Netherlands Media print
Document type Journal Article
PubMed
9099893
DOI
10.1016/s0378-1119(96)00767-6
PII: S0378-1119(96)00767-6
Knihovny.cz E-resources
- MeSH
- Bacillus subtilis virology MeSH
- DNA-Directed RNA Polymerases metabolism ultrastructure MeSH
- DNA, Viral * MeSH
- Single-Strand Specific DNA and RNA Endonucleases metabolism MeSH
- Gene Expression MeSH
- Bacillus Phages genetics MeSH
- Transcription, Genetic * MeSH
- Cloning, Molecular MeSH
- Chromosome Mapping MeSH
- Molecular Sequence Data MeSH
- Plasmids ultrastructure MeSH
- Computer Simulation * MeSH
- Promoter Regions, Genetic * MeSH
- Tetracycline Resistance genetics MeSH
- Base Sequence MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA-Directed RNA Polymerases MeSH
- DNA, Viral * MeSH
- Single-Strand Specific DNA and RNA Endonucleases MeSH
A fragment (172 bp) of B. subtilis phage phi29 DNA, which does not contain a functional promoter for phage transcription, has been shown to direct transcription in the promoter-probe plasmid pPV33. The promoter candidate found in this fragment by the computer method of acceptability is compared with cryptic promoters selected by this computer method. It is characterized in vitro by electron microscopic visualization of RNA polymerase binding and 'run off' transcription, and in vivo by high resolution S1 mapping.
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