Identification of procaryotic developmental stages by statistical analyses of two-dimensional gel patterns
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Electrophoresis, Gel, Two-Dimensional statistics & numerical data MeSH
- Algorithms MeSH
- Bacterial Proteins genetics isolation & purification MeSH
- Databases, Factual MeSH
- Genome, Bacterial MeSH
- Data Interpretation, Statistical MeSH
- Multivariate Analysis MeSH
- Peptide Mapping statistics & numerical data MeSH
- Prokaryotic Cells MeSH
- Cluster Analysis MeSH
- Streptomyces chemistry genetics growth & development MeSH
- Gene Expression Regulation, Developmental MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
Multivariate statistical comparisons of two-dimensional protein (2-D) gel patterns were used for the first time to define stages of a biological developmental system. The differentiating procaryote, Streptomyces coelicolor, was radiolabeled in liquid cultures at 16 intervals during development, and radioactive proteins were separated and quantified on 2-D gels. Cluster, principal component, and correlation analyses classified these gel patterns into four distinct groups, each reflecting a pattern of gene expression specific for a stage of development. These studies focused our attention on a phase of arrested growth as a key regulatory transition leading to secondary metabolism and a phase of renewed growth. Proteins whose synthesis was switched on or off during the "transitional" phase (some 21 and 18, respectively) were identified and will be the focus of future studies designed to identify their physiological or regulatory function.
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General and molecular microbiology and microbial genetics in the IM CAS
