Electron microscopic demonstration of the penetration of liposomes through skin
Language English Country Czech Republic Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
9338124
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- Microscopy, Electron methods MeSH
- Endocytosis MeSH
- Keratinocytes metabolism MeSH
- Rabbits MeSH
- Skin drug effects metabolism ultrastructure MeSH
- Humans MeSH
- Liposomes metabolism pharmacokinetics MeSH
- Macrophages metabolism MeSH
- Guinea Pigs MeSH
- Vitamin A pharmacology MeSH
- Hair Follicle metabolism MeSH
- Gold metabolism MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Humans MeSH
- Guinea Pigs MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Liposomes MeSH
- Vitamin A MeSH
- Gold MeSH
To verify the penetration of liposomes through skin, we have used liposomes with encapsulated protein G-gold conjugate in a gel vehicle. Skin samples were examined 2, 4, 24, 48, and 72 h after liposome application. Our findings show that the penetration of liposomes through skin depends mainly on their size. Liposomes up to 600 nm in diameter penetrate through skin rather easily, whereas liposomes 1000 nm and more in diameter remain interiorized in the stratum corneum. The main penetration of liposomes proceeds along the hair sheaths as indicated by larger amounts of free liposomes in the corium of guinea pigs.
