Isolation and characterization of a new dsRNA virus from Wickerhamia fluorescens
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9449770
DOI
10.1007/bf02814620
Knihovny.cz E-resources
- MeSH
- RNA, Double-Stranded isolation & purification MeSH
- Electrophoresis, Agar Gel MeSH
- Capsid isolation & purification MeSH
- Yeasts virology MeSH
- Molecular Weight MeSH
- RNA, Viral isolation & purification MeSH
- Totivirus genetics isolation & purification MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- RNA, Double-Stranded MeSH
- RNA, Viral MeSH
Virus-like particles (VLPs) were isolated from the yeast Wickerhamia fluorescens strain CCY61-1-1. The VLPs are approximately 42 nm in diameter and contain only one species of dsRNA molecule. The apparent length of the dsRNA determined by native agarose gel electrophoresis was 4.6 kbp. Analysis of protein content of the VLPs showed them to contain one major capsid protein with an apparent molar mass of 74.5 kDa.
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