Lack of surface receptors not restriction-modification system determines F4 phage resistance in Streptococcus bovis II/1
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9569625
DOI
10.1007/bf02815538
Knihovny.cz E-zdroje
- MeSH
- DNA restrikčně-modifikační enzymy * MeSH
- DNA virů izolace a purifikace MeSH
- fágy streptokoků růst a vývoj ultrastruktura MeSH
- Streptococcus bovis ultrastruktura virologie MeSH
- virové receptory * MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA restrikčně-modifikační enzymy * MeSH
- DNA virů MeSH
- virové receptory * MeSH
The resistance of Streptococcus bovis strain II/1, the producer of SbvI restriction endonuclease, to F4 phage infection was demonstrated by the double-agar-layer method. Despite the presence of restriction endonuclease SbvI which can cleave F4 phage DNA to numerous fragments in vitro, the evidence that adsorption inhibition is the most important defence mechanism in phage resistance of S. bovis II/1 strain was obtained by adhesion experiments in vivo. Electron microscopy of phage-host mixtures showed many phage particles on the bacterial surface of phage-sensitive S. bovis 47/3 control strain in comparison with no phage particles seen on S. bovis II/1 (phage-resistant) strain surface.
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