Antitumor action of bovine seminal ribonuclease. Cytostatic effect on human melanoma and mouse seminoma
Language English Country Slovakia Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9604999
Knihovny.cz E-resources
- MeSH
- Endoribonucleases therapeutic use MeSH
- Humans MeSH
- Melanoma drug therapy MeSH
- Mice, Nude MeSH
- Mice MeSH
- Tumor Cells, Cultured MeSH
- Ribonuclease, Pancreatic therapeutic use MeSH
- Antineoplastic Agents therapeutic use MeSH
- Drug Screening Assays, Antitumor MeSH
- Seminoma drug therapy MeSH
- Testicular Neoplasms drug therapy MeSH
- Cell Survival drug effects MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Endoribonucleases MeSH
- Ribonuclease, Pancreatic MeSH
- Antineoplastic Agents MeSH
- ribonuclease SPL MeSH Browser
This paper reports on the antitumor activity of BS RNase on human melanoma and mouse seminoma. Human melanoma cells established in culture were extremely susceptible to BS RNase, administered in concentrations ranging from 1-100 microg/ml. Concentrations of BS RNase over 10 microg/ml caused complete inhibition of cell growth. Bovine pancreatic ribonuclease (RNase A), a prototype of the ribonuclease superfamily, did not exert any effect under these conditions. Based on our previous results, athymic mice bearing human melanoma or mouse seminoma were treated with intratumoral administration of BS RNase (12.5 mg/kg b.w.). This dose was injected for five consecutive days excluding weekends. The intratumoral administration of BS RNase to nude mice bearing human melanoma showed a significant antitumor effect. There were no tumors seen in eighty percent of mice treated for three weeks, and tumors in the other mice diminished significantly. After some delay the tumors started to regrow. Prolonging of the treatment to five weeks had a similar effect. The effect of BS RNase on mouse seminoma was well pronounced. Five to seven doses of BS RNase were sufficient to eliminate tumors in all treated mice. However, as in the previous experiment, the growth of tumor tissue later reappeared.