Apoptotic damage of DNA in human leukaemic HL-60 cells treated with C2-ceramide was detected after G1 blockade of the cell cycle
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9727507
Knihovny.cz E-zdroje
- MeSH
- apoptóza účinky léků MeSH
- buněčný cyklus účinky léků MeSH
- G1 fáze účinky léků MeSH
- HL-60 buňky MeSH
- inhibitory enzymů farmakologie MeSH
- lidé MeSH
- poškození DNA účinky léků MeSH
- průtoková cytometrie MeSH
- separace buněk MeSH
- sfingosin analogy a deriváty farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- inhibitory enzymů MeSH
- N-acetylsphingosine MeSH Prohlížeč
- sfingosin MeSH
Apoptosis was induced by treatment of HL-60 cells with C2-ceramide. Apoptotic damage of DNA was detected according to the sub-G1 peak on a flow cytometer, according to the typical morphology and according to the DNA fragmentation "ladder" after gel electrophoresis. It was shown that the apoptotic cleavage followed after G1 blockade of the cell cycle. A high correlation coefficient (rs=0.957) was found between the percentages of G1 blocked cells and apoptotic cells. This high correlation together with the appearance of the sub-G1 peak suggests that the G1 blocked HL-60 cells were subject to apoptotic death. It is deduced that the mechanisms leading to G1 blockade of the cell cycle and activation of apoptosis in HL-60 cells are interconnected.
Cytogenetics and cytology of retinoblastomas
