Differences in enterocyte brush border enzyme activities in ageing rats reared in germ-free and conventional conditions
Jazyk angličtina Země Česko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
9803471
Knihovny.cz E-zdroje
- MeSH
- beta-galaktosidasa metabolismus MeSH
- dipeptidylpeptidasa 4 metabolismus MeSH
- disacharidasy metabolismus MeSH
- glukoamylasa metabolismus MeSH
- gnotobiologické modely * MeSH
- jejunum enzymologie ultrastruktura MeSH
- krysa rodu Rattus MeSH
- laktasa MeSH
- mikroklky enzymologie MeSH
- potkani Wistar MeSH
- sacharasa metabolismus MeSH
- stárnutí * MeSH
- střevní sliznice enzymologie ultrastruktura MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- beta-galaktosidasa MeSH
- dipeptidylpeptidasa 4 MeSH
- disacharidasy MeSH
- glukoamylasa MeSH
- laktasa MeSH
- sacharasa MeSH
The aim of this study was to evaluate the levels of disaccharidase and dipeptidyl peptidase i.v. activities in rat jejunal enterocytes under the influence of long-term germ-free conditions. We found that the brush-border lactase and dipeptidyl peptidase i.v. activities were two to three times higher in 2-month-old germ-free rats in comparison with their conventional counterparts. The highest effect of germ-free condition was observed on lactase activity in 6-month-old and dipeptidyl peptidase i.v. in 2-month-old rats. No difference between germ-free and conventional rats in sucrase and glucoamylase activities was found in 2-month-old rats. The difference develops with increasing age, sucrase activity becoming significantly higher in 6- and 12-month-old rats and glucoamylase in 12-month-old germ-free rats.
