An integron of class 1 is present on the plasmid pCG4 from gram-positive bacterium Corynebacterium glutamicum
Language English Country England, Great Britain Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
9868786
DOI
10.1111/j.1574-6968.1998.tb13345.x
PII: S0378-1097(98)00516-3
Knihovny.cz E-resources
- MeSH
- Drug Resistance, Microbial MeSH
- Bacterial Proteins genetics MeSH
- Corynebacterium drug effects genetics MeSH
- Escherichia coli genetics MeSH
- Integrases genetics MeSH
- Molecular Sequence Data MeSH
- Plasmids genetics MeSH
- Pseudomonas aeruginosa genetics MeSH
- Base Sequence MeSH
- Streptomycin pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Bacterial Proteins MeSH
- Integrases MeSH
- Streptomycin MeSH
The streptomycin/spectinomycin resistance determinant of the 29-kb plasmid pCG4 from Corynebacterium glutamicum was found to be a part of a typical class 1 integron. The sequence analysis revealed that the integron (designated InCg) identified in this Gram-positive bacterium is almost identical to the integron InC present on the plasmid pSA1700 from the Gram-negative bacterium Pseudomonas aeruginosa. Differences in only two base pairs were found in the 3.8-kb sequence. One base substitution (G-->C) is present in the streptomycin/spectinomycin resistance determinant which is thus identical to the aadA2a gene from the integron In6 of the broad-host-range plasmid pSa. The other one (C-->G) is present in the extended -10 region of the integron promoter involved in expression of the antibiotic resistance gene. It was shown that this novel version of the integron promoter displays five times higher activity in both C. glutamicum and Escherichia coli than the original one.
References provided by Crossref.org
General and molecular microbiology and microbial genetics in the IM CAS
Control of rep gene expression in plasmid pGA1 from Corynebacterium glutamicum
Analysis of the Corynebacterium glutamicum dapA promoter
GENBANK
Y14748, Y17696
