An integron of class 1 is present on the plasmid pCG4 from gram-positive bacterium Corynebacterium glutamicum
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
9868786
DOI
10.1111/j.1574-6968.1998.tb13345.x
PII: S0378-1097(98)00516-3
Knihovny.cz E-zdroje
- MeSH
- antibiotická rezistence MeSH
- bakteriální proteiny genetika MeSH
- Corynebacterium účinky léků genetika MeSH
- Escherichia coli genetika MeSH
- integrasy genetika MeSH
- molekulární sekvence - údaje MeSH
- plazmidy genetika MeSH
- Pseudomonas aeruginosa genetika MeSH
- sekvence nukleotidů MeSH
- streptomycin farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- bakteriální proteiny MeSH
- integrasy MeSH
- streptomycin MeSH
The streptomycin/spectinomycin resistance determinant of the 29-kb plasmid pCG4 from Corynebacterium glutamicum was found to be a part of a typical class 1 integron. The sequence analysis revealed that the integron (designated InCg) identified in this Gram-positive bacterium is almost identical to the integron InC present on the plasmid pSA1700 from the Gram-negative bacterium Pseudomonas aeruginosa. Differences in only two base pairs were found in the 3.8-kb sequence. One base substitution (G-->C) is present in the streptomycin/spectinomycin resistance determinant which is thus identical to the aadA2a gene from the integron In6 of the broad-host-range plasmid pSa. The other one (C-->G) is present in the extended -10 region of the integron promoter involved in expression of the antibiotic resistance gene. It was shown that this novel version of the integron promoter displays five times higher activity in both C. glutamicum and Escherichia coli than the original one.
Citace poskytuje Crossref.org
General and molecular microbiology and microbial genetics in the IM CAS
Control of rep gene expression in plasmid pGA1 from Corynebacterium glutamicum
Analysis of the Corynebacterium glutamicum dapA promoter
GENBANK
Y14748, Y17696
