Investigation into PCB biodegradation using uniformly 14C-labelled dichlorobiphenyl
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
- MeSH
- Biodegradation, Environmental MeSH
- Biotransformation MeSH
- Kinetics MeSH
- Soil Pollutants metabolism pharmacokinetics MeSH
- Pleurotus metabolism MeSH
- Gas Chromatography-Mass Spectrometry MeSH
- Polychlorinated Biphenyls metabolism pharmacokinetics MeSH
- Pseudomonas putida metabolism MeSH
- Pseudomonas metabolism MeSH
- Carbon Radioisotopes MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- 3,3'-dichlorobiphenyl MeSH Browser
- Soil Pollutants MeSH
- Polychlorinated Biphenyls MeSH
- Carbon Radioisotopes MeSH
Biodegradation of polychlorinated biphenyls (PCBs) in soil is considered to be very complex due to various physico-chemical factors involved. Isotope labelling technique is the best to trace fate of the xenobiotic in the environment. In this work, the uniformly 14C-labelled PCB congener 11 (3,3'-chlorobiphenyl) was chosen as a low chlorinated coplanar biphenyl which was assumed to be readily degraded by microorganisms. Pleurotus ostreatus and two Pseudomonas species, representing white rot fungi and soil bacteria were used separately or in a consortium. The amount of liberated 14CO2 and radio-HPLC, HPLC, GC-MS, and radio-TLC analyses of extracts at the end of a two-month experiment showed that the mineralization of PCB 11 was < 0.4%, volatilization < 3.1%, and 30% of radioactivity was irreversibly bound to the soil matrix. The respective contents of all intermediate metabolites were 4.7 to 10.5 and 2.5 to 2.7% where Pseudomonas alcaligenes alone or in combination with P. putida was applied. 3-Chlorobenzoic acid was the major biodegradation product.
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