Butyrolactone I reversibly inhibits meiotic maturation of bovine oocytes,Without influencing chromosome condensation activity
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, P.H.S.
Grantová podpora
HD22681
NICHD NIH HHS - United States
- MeSH
- chromatin ultrastruktura MeSH
- chromozomy účinky léků MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- encefalitogenní základní proteiny metabolismus MeSH
- fertilizace fyziologie MeSH
- fixace tkání MeSH
- folikulární tekutina cytologie MeSH
- gama-butyrolakton analogy a deriváty farmakologie MeSH
- inhibitory enzymů farmakologie MeSH
- kultivační média MeSH
- kultivované buňky MeSH
- meióza účinky léků MeSH
- mitogenem aktivované proteinkinasy antagonisté a inhibitory metabolismus MeSH
- oocyty účinky léků enzymologie růst a vývoj MeSH
- proteinkinasa CDC2 metabolismus MeSH
- proteinkinasy metabolismus MeSH
- skot MeSH
- spermie fyziologie MeSH
- western blotting MeSH
- zárodečné centrum lymfatické uzliny účinky léků MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Názvy látek
- butyrolactone I MeSH Prohlížeč
- chromatin MeSH
- encefalitogenní základní proteiny MeSH
- gama-butyrolakton MeSH
- histone H1 kinase MeSH Prohlížeč
- inhibitory enzymů MeSH
- kultivační média MeSH
- mitogenem aktivované proteinkinasy MeSH
- proteinkinasa CDC2 MeSH
- proteinkinasy MeSH
In this study, butyrolactone I (BL I), a potent and specific inhibitor of cyclin-dependent kinases, was shown to block germinal vesicle (GV) breakdown (GVBD) in bovine oocytes in a concentration-dependent manner; GVBD was almost totally inhibited over the course of 24-48 h of culture when 100 microM BL I was included in tissue culture medium 199 containing either polyvinyl alcohol or BSA. Correlated with this inhibition was the failure of either p34(cdc2) kinase or mitogen-activated protein (MAP) kinase to become activated, and it was unlikely that BL I directly inhibited MAP kinase, since 100 microM BL I did not inhibit MAP kinase activity present in extracts obtained from metaphase II-arrested bovine eggs that possess high levels of MAP kinase activity. Nevertheless, the formation of highly condensed bivalents was observed in 78% of the BL I-treated GV-intact oocytes. This result suggests that chromosome condensation during first meiosis in bovine oocytes does not require the activity of either p34(cdc2) kinase or MAP kinase. Treatment of BL I-arrested oocytes with okadaic acid (OA) did not result in either the activation of p34(cdc2) kinase or MAP kinase, or inducement of GVBD. The BL I-induced block of GVBD for 24 h was reversible, and a subsequent 24-h culture resulted in 90% of oocytes reaching metaphase II with emission of the first polar body. Correlated with the progression to and arrest at metaphase II was the full activation of both p34(cdc2) and MAP kinases. The reversibility after 48 h of culture in BL I was partially decreased when compared to that achieved after an initial 24-h culture. Fertilization in vitro of these eggs resulted in a high incidence of both sperm penetration and pronucleus formation (88% and 70%, respectively).
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