Dynamics of beta-glucosidase Zm-p60.1 ectopic expression during transgenic pollen development: a histochemical approach
Language English Country Slovakia Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10707855
Knihovny.cz E-resources
- MeSH
- beta-Glucosidase genetics MeSH
- DNA Primers genetics MeSH
- Gene Expression MeSH
- Plants, Genetically Modified MeSH
- Plants, Toxic MeSH
- Zea mays enzymology genetics growth & development MeSH
- RNA, Messenger genetics metabolism MeSH
- Pollen enzymology genetics growth & development MeSH
- RNA, Plant genetics metabolism MeSH
- Base Sequence MeSH
- Nicotiana MeSH
- Transformation, Genetic MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- beta-Glucosidase MeSH
- cytokinin-beta-glucosidase MeSH Browser
- DNA Primers MeSH
- RNA, Messenger MeSH
- RNA, Plant MeSH
Zm-p60.1 is maize cDNA coding cytokinin-glucoside specific beta-glucosidase. Indigogenic method was used for histochemical localization of Zm-p60.1 beta-glucosidase activity in various developmental stages of transgenic tobacco anthers. Expression of Zm-p60.1 cDNA in T7 tobacco plants is controlled by the CaMV 35S promoter. Another type of tobacco transformant expresses Zm-p60.1 under the control of LAT 52 promoter. Histochemical detection has proved different patterns of beta-glucosidase activity during tobacco pollen development in these two types of transformants. Zm-p60.1 beta-glucosidase activity had not direct influence on pollen germinability.
