Regulation of Na+, K+-ATPase density by the extracellular ionic and osmotic environment in bovine articular chondrocytes
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
Wellcome Trust - United Kingdom
PubMed
10783917
Knihovny.cz E-resources
- MeSH
- Enzyme Activation drug effects physiology MeSH
- Chondrocytes cytology enzymology MeSH
- Extracellular Space metabolism MeSH
- Hypertonic Solutions pharmacology MeSH
- Isotonic Solutions pharmacology MeSH
- Cartilage, Articular cytology enzymology MeSH
- Culture Media pharmacology MeSH
- Cells, Cultured MeSH
- Osmotic Pressure MeSH
- Signal Transduction physiology MeSH
- Cattle MeSH
- Sodium-Potassium-Exchanging ATPase metabolism MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Hypertonic Solutions MeSH
- Isotonic Solutions MeSH
- Culture Media MeSH
- Sodium-Potassium-Exchanging ATPase MeSH
The abundance of Na+, K+-ATPase in cartilage is controlled by the ionic composition of the extracellular environment of chondrocytes, and specifically depends on the local concentration of polyanionic matrix proteoglycans. In this study, it was found that the plasma membrane density of Na+, K+-ATPase in isolated chondrocytes is sensitive to both ionic and osmotic changes in the extracellular environment. The upregulation observed experimentally was similar in magnitude as measured by 3H-ouabain binding, which indicates that chondrocytes respond adaptively to both ionic and osmotic stimuli. The precise mechanism for this novel mode of Na+, K+-ATPase regulation has yet to be elucidated. Physiological perturbation of the ionic and osmotic environment of chondrocytes may alter intracellular Na+ concentration and this may be one of a number of stimuli responsible for alterations to the expression and plasma membrane abundance of Na+, K+-ATPase in the cells.
