Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10802051
DOI
10.1016/s0014-5793(00)01488-5
PII: S0014-5793(00)01488-5
Knihovny.cz E-resources
- MeSH
- Biomarkers analysis MeSH
- Cell Differentiation * drug effects MeSH
- Time Factors MeSH
- Protein Denaturation MeSH
- Dimethyl Sulfoxide pharmacology MeSH
- Carcinoma, Embryonal metabolism pathology MeSH
- Fluorescent Antibody Technique MeSH
- Microtubules drug effects metabolism MeSH
- Molecular Weight MeSH
- Mice MeSH
- Tumor Cells, Cultured MeSH
- Neurites drug effects MeSH
- Precipitin Tests MeSH
- Microtubule-Associated Proteins chemistry immunology metabolism MeSH
- Antibodies immunology MeSH
- Tretinoin pharmacology MeSH
- Hot Temperature MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- Dimethyl Sulfoxide MeSH
- Microtubule-Associated Proteins MeSH
- Antibodies MeSH
- Tretinoin MeSH
The MA-01 antigen, a thermolabile 210 kDa microtubule-interacting protein, is present in P19 embryonal carcinoma cells on microtubular structures as well as in cytosol. After aggregation of the cells and subsequent incubation with all-trans-retinoic acid (RA), the level of MA-01 expression increased approximately 10 times during 15 days. The increase started after 2 days of incubation with RA and preceded the appearance of neuron-specific tubulin betaIII, MAP2C and neurofilaments. Such elevated expression of MA-01 antigen was not detected in P19 cells treated with dimethylsulfoxide. These data indicate that enhanced expression of MA-01 antigen is one of the earliest events occurring in P19 cells during neuronal differentiation.
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