Molecular characterization of a calmodulin-like dictyostelium protein CalB
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10818233
DOI
10.1016/s0014-5793(00)01551-9
PII: S0014-5793(00)01551-9
Knihovny.cz E-resources
- MeSH
- Dictyostelium MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Immunoblotting MeSH
- Calmodulin genetics MeSH
- Cloning, Molecular MeSH
- Cells, Cultured MeSH
- Molecular Sequence Data MeSH
- Calcium-Binding Proteins genetics metabolism MeSH
- Protozoan Proteins * MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Sequence Alignment MeSH
- Calcium metabolism MeSH
- Protein Binding MeSH
- Gene Expression Regulation, Developmental MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- CalB protein, Dictyostelium MeSH Browser
- Calmodulin MeSH
- Calcium-Binding Proteins MeSH
- Protozoan Proteins * MeSH
- Calcium MeSH
A gene named calB was cloned and characterized in Dictyostelium. A relationship to calmodulin (CaM) is suggested by sequence identity (50%), similar exon-intron structure and cross-reactivity with anti-CaM sera. The level of calB mRNA is developmentally regulated with maxima during aggregation and in spores. CalB null cells grow normally, develop and produce viable spores. We demonstrated the capacity of tagged CalB to bind Ca(2+) using the (45)Ca(2+) overlay assay and showed that its mobility on SDS-PAGE is dependent on Ca(2+)/EGTA pretreatment.
Department of Physiology and Developmental Biology Charles University Vinicná 7 Praha Czech Republic
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GENBANK
AF001981
