Highly conserved DNA sequence present in small plasmids from Selenomonas ruminantium
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10873531
DOI
10.1006/plas.2000.1464
PII: S0147-619X(00)91464-1
Knihovny.cz E-resources
- MeSH
- DNA, Bacterial * MeSH
- Conserved Sequence * MeSH
- Molecular Sequence Data MeSH
- Plasmids * MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA methods MeSH
- Selenomonas genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Bacterial * MeSH
Plasmid pJW1 from Selenomonas ruminantium subsp. lactilytica strain JW13 has been cloned in Escherichia coli vector pBluescriptSK(-) and completely sequenced. The plasmid is only 1410 bp with an overall GC content of 42.2%. Computer analysis of sequence data revealed a single open reading frame (ORF1, 146 amino acids, MW 16,525.5 Da) encoding a putative replication protein which is similar to the Rep protein of Ruminobacter amylophilus plasmid pRAO1. ORF1 is followed by a long AT-rich (75%) region and a region abundant in direct and inverted repeats. Comparison of DNA sequences revealed the presence of a short (<250 bp) DNA segment which is highly conserved between several small S. ruminantium plasmids including pJDB21.
References provided by Crossref.org
Spreading and mutability of Selenomonas ruminantium plasmids
Plasmids of Selenomonas ruminantium and development of host-vector system
GENBANK
AF141869
