Characterization of mammalian orthologues of the Drosophila osa gene: cDNA cloning, expression, chromosomal localization, and direct physical interaction with Brahma chromatin-remodeling complex
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11318604
DOI
10.1006/geno.2001.6477
PII: S0888754301964778
Knihovny.cz E-zdroje
- MeSH
- alternativní sestřih MeSH
- buněčné jádro metabolismus MeSH
- buněčné linie MeSH
- chromatin fyziologie MeSH
- DNA vazebné proteiny genetika MeSH
- Drosophila melanogaster MeSH
- genetická transkripce MeSH
- jaderné proteiny genetika MeSH
- konzervovaná sekvence MeSH
- lidé MeSH
- lidské chromozomy, pár 1 MeSH
- molekulární evoluce MeSH
- molekulární sekvence - údaje MeSH
- myši MeSH
- proteiny Drosophily * MeSH
- regulační oblasti nukleových kyselin MeSH
- sekvence aminokyselin MeSH
- sekvenční seřazení MeSH
- transkripční faktory MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- Arid1a protein, mouse MeSH Prohlížeč
- ARID1B protein, human MeSH Prohlížeč
- chromatin MeSH
- DNA vazebné proteiny MeSH
- jaderné proteiny MeSH
- osa protein, Drosophila MeSH Prohlížeč
- proteiny Drosophily * MeSH
- transkripční faktory MeSH
The osa gene of Drosophila melanogaster encodes a nuclear protein that is a component of the Brahma chromatin-remodeling complex. Osa is required for embryonic segmentation, development of the notum and wing margin, and photoreceptor differentiation. In these tissues, osa mutations have effects opposite to those caused by wingless (wg) mutations, suggesting that osa functions as an antagonist of wg signaling. Here we describe the cloning and characterization of mammalian orthologues of osa. Three evolutionarily conserved domains were identified in Osa family members: the N-terminal Bright domain and C-terminally located Osa homology domains 1 and 2. RNase protection analysis indicates a widespread expression of the Osa1 gene during mouse development, in adult tissues, and in cultured cell lines. The Osa1 gene was localized to mouse chromosome 4, within the region syntenic to chromosomal position 1p35-p36 of its human counterpart. We present evidence that the OSA1 product is localized in the nucleus and associates with human Brahma complex, which suggests evolutionarily conserved function for Osa in gene regulation between mammals and Drosophila.
Citace poskytuje Crossref.org
GENBANK
AF268912, AF268913
