Monoclonal antibodies to intra-acrosomal proteins inhibit gamete binding in vitro
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11480614
DOI
10.1016/s0093-691x(01)00557-x
PII: S0093-691X(01)00557-X
Knihovny.cz E-resources
- MeSH
- Acrosin immunology MeSH
- Acrosome immunology MeSH
- Sperm-Ovum Interactions immunology MeSH
- Antibodies, Monoclonal immunology MeSH
- Swine MeSH
- In Vitro Techniques MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Acrosin MeSH
- Antibodies, Monoclonal MeSH
The sperm-zona pellucida-binding assay in vitro was used as a functional test for zona pellucida-binding ability of boar spermatozoa after co-incubation with monoclonal antibodies against intra-acrosomal proteins. The effect of monoclonal antibodies ACR.2 against boar acrosin (55, 53, 45 and 38 kDa), and Hs-8 against boar intra-acrosomal protein (230, 110, 88, 60, 48 kDa) on boar spermatozoa-porcine oocyte binding was examined. The sperm-zona pellucida-binding was reduced when medium was supplemented with monoclonal antibodies during sperm-oocyte co-incubation, but not when capacitated spermatozoa were pretreated with monoclonal antibodies before incubation with oocytes. Our results show that the monoclonal antibodies (ACR.2, Hs-8) against intra-acrosomal proteins reduce the secondary sperm-zona pellucida-binding with statistically significant difference. This suggests the role of these proteins in the early phases of fertilization.
References provided by Crossref.org
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