The aim of this study was to analyse the multigenerational effects of para-nonylphenol (NP) and resveratrol (RES) on the body weight, organ weight and reproductive fitness of outbred CD-1 mice. The data indicate that in male mice, NP had an effect on the weight of selected reproductive organs and the kidneys in the parental (P) generation males. Effects on selected reproductive organs, the liver and kidneys in the F1-generation males were also seen. In females, effects of NP on body weight and kidney weight were seen in the P generation, but no effects on any measured parameter were seen in the F1 generation. RES had no effect on body weight but did have some effect on selected male and female reproductive organs in the P generation. RES altered the spleen and liver weights of P-generation males and the kidney weight of F1-generation males. Acrosomal integrity (using a monoclonal antibody against intra-acrosomal sperm proteins) was assessed for both generations of NP- and RES-treated mice. A significant reduction in acrosomal integrity was seen in both generations of NP-treated, but not in RES-treated, mice. Fewer offspring were observed in the second litter of the F2 generation of mice treated with NP; no similar effect was seen in RES-treated mice. The litter sex ratio was not different from controls. Unlike RES, NP had a negative effect on spermatogenesis and sperm quality with a resultant impact on in vivo fertility.

Department of Biology and Biochemistry of Fertilization Institute of Molecular Genetics Academy of Sciences of the Czech Republic Videnska 1083 142 20 Prague 4 Czech Republic

Zobrazit více v PubMed

Mantovani A. Xenobiotics with potential endocrine activities ("endocrine disruptors"): an emerging public health problem. 2002.

Mantovani A. Definizione di limiti massimi di residui negli alimenti: criteri per assicurare una adeguata protezione nei confronti dellínfazia. Notiziario dellÍnstituto Superiore di Sanita. 1999;12:5–8.

Ahel M, McEvoy J, Giger W. Bioaccumulation of the lipophilic metabolites of nonionic surfactants in freshwater organisms. Environ Pollut. 1993;79:243–248. doi: 10.1016/0269-7491(93)90096-7. PubMed DOI

Lee PC. Disruption of male reproductive tract development by administration of the xenoestrogen, nonylphenol, to male newborn rats. Endocrine. 1998;9:105–111. doi: 10.1385/ENDO:9:1:105. PubMed DOI

De Jager C, Bornman MS, Wandrag S, van der Horst G. I. Effect of p-nonylphenol, an environmental toxicant with oestrogenic properties, on fertility potentiall in adult male rats. Andrologia. 1999;31:99–106. doi: 10.1046/j.1439-0272.1999.00245.x. PubMed DOI

De Jager C, Bornman MS, Oosthuizen MC. II. The effect of p-nonylphenol on the fertility potential of male rats after gestational, lactational and direct exposure. Andrologia. 1999;31:107–113. doi: 10.1046/j.1439-0272.1999.00246.x. PubMed DOI

De Jager C, Bornman MS, Wandrag S, Sharp VW. The lethal dose and potential reproductive effects of p-nonylphenol in rats: a preliminary study. Arch Androl. 2001;46:183–187. doi: 10.1080/01485010151096441. PubMed DOI

Odum J, Ashby J. Neonatal exposure of male rats to nonylphenol has no effect on the reproductive tract. Toxicol Science. 2000;56:400–404. doi: 10.1093/toxsci/56.2.400. PubMed DOI

Landcake P, Price RJ. The production of resveratrol by Vitis vinifera and other members of the Vitacea as a response to infection or injury. Physiol Plant Pathol. 1976;9:77–86.

Siemann GJ, Creasy LL. Concentration of the phytoalexin resveratrol in wine. American Journal of Ecology and Viticulture. 1992;43:49–52.

Melzoch K, Filip V, Buckiova D, Hanzlikova I, Smidrkal J. Resveratrol – occurrence in wine originating from Czech vineyard regions and effect on human health. Czech J Food Sci. 2000;18:35–40.

Savouret JF, Quesne M. Resveratrol and cancer: a review. Biomed Pharmacother. 2002;56:84–87. doi: 10.1016/S0753-3322(01)00158-5. PubMed DOI

Gehm BD, McAndrews JM, Chien PY, Jameson JL. Resveratrol, a polyphenolic compound found in grapes and wine, is an agonist for the estrogen receptor. Proc Natl Acad Sci U S A. 1997;94:14138–14143. doi: 10.1073/pnas.94.25.14138. PubMed DOI PMC

Ashby J, Tinwell H, Pennie W, Brooks AN, Lefevre PA, Beresford N, Sumpter JP. Partial and weak oestrogenicity of the red wine constituent resverstrol: consideration of its superagonist activity in MCF-7 cells and its suggested cardiovascular protective effect. J Appl Toxicol. 1999;19:39–45. doi: 10.1002/(SICI)1099-1263(199901/02)19:1<39::AID-JAT534>3.0.CO;2-M. PubMed DOI

Peknicova J, Kyselova V, Buckiova D, Boubelik M. Effect of an endocrine disruptor on mammalian fertility. Application of monoclonal antibodies against sperm proteins as markers for testing sperm damage. Amer J Reprod Immunol. 2002;47:311–318. doi: 10.1034/j.1600-0897.2002.01112.x. PubMed DOI

Peknicova J, Moos J. Monoclonal antibodies against boar acrosomal antigens labeling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia. 1990;22:427–435. PubMed

Peknicova J, Moos J, Mollova M, Srsen V, Capkova J. Changes in immunochemical localization of acrosomal and sperm proteins in boar spermatozoa during capacitation and induced acrosome reaction. Anim Reprod Sci. 1994;35:255–271. doi: 10.1016/0378-4320(94)90041-8. DOI

Peknicova J, Capkova J, Geussova G, Ivanova M, Mollova M. Monoclonal antibodies to intra-acrosomal protein inhibit gamete binding in vitro. Theriogenology. 2001;56:211–223. doi: 10.1016/S0093-691X(01)00557-X. PubMed DOI

Peknicova J, Moos J. Antibody against 28 kDa intra-acrosomal sperm proteins as a tool for evaluation of acrosomal integrity in bull spermatozoa. Folia Biol (Praha) 1998;44:93–96. PubMed

Chladek D, Peknicova J, Capkova J, Geussova G, Tepla O, Madar J. Application of monoclonal antibodies against sperm proteins to diagnostics of human pathology and for selection of suitable method in assisted reproduction. Czech Gynaecol. 2000;65:28–32. PubMed

Sutovsky P, Terada Y, Schateen G. Ubiquitin-based sperm assay for the diagnosis of the male factor infertility. Hum Reprod. 2001;15:2610–2620. PubMed

Ramalho-Santos J, Terada Y, Schatten G. VAMP/synaptobrevin as an acrosomal marker for human sperm. Fertil Steril. 2002;77:159–161. doi: 10.1016/S0015-0282(01)02957-0. PubMed DOI

Skakkebaek NE, Rajpert E, De Meyts, Main M. Testicular dysgenesis syndrome: an increasingly common developmental disorder with environmental aspects. Human Reprod. 2001;16:972–978. doi: 10.1093/humrep/16.5.972. PubMed DOI

Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm