Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test
Jazyk angličtina Země Německo Médium print
Typ dokumentu časopisecké články
- MeSH
- akrosin imunologie MeSH
- akrozom imunologie MeSH
- antigeny izolace a purifikace MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- fluorescenční protilátková technika MeSH
- monoklonální protilátky * MeSH
- prasata MeSH
- proteiny imunologie MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- akrosin MeSH
- antigeny MeSH
- monoklonální protilátky * MeSH
- proteiny MeSH
Monoclonal antibodies specific for acrosomal proteins were prepared and characterized. Two of these monoclonal antibodies, ACR.3 and ACR.11, reacted with the same molecular specificity of acrosomal antigens (18-20 kDa), antibody ACR.4 reacted with the 25-27 kDa antigens, and antibody ACR.2 reacted with several forms of boar acrosin (55, 53, 45, and 38 kDa). All monoclonal antibodies labelled the acrosomes of undamaged spermatozoa in immunofluorescence test. This test should be convenient as an immunological test of the sperm quality.
Citace poskytuje Crossref.org
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