Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test
Language English Country Germany Media print
Document type Journal Article
- MeSH
- Acrosin immunology MeSH
- Acrosome immunology MeSH
- Antigens isolation & purification MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Fluorescent Antibody Technique MeSH
- Antibodies, Monoclonal * MeSH
- Swine MeSH
- Proteins immunology MeSH
- Blotting, Western MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Acrosin MeSH
- Antigens MeSH
- Antibodies, Monoclonal * MeSH
- Proteins MeSH
Monoclonal antibodies specific for acrosomal proteins were prepared and characterized. Two of these monoclonal antibodies, ACR.3 and ACR.11, reacted with the same molecular specificity of acrosomal antigens (18-20 kDa), antibody ACR.4 reacted with the 25-27 kDa antigens, and antibody ACR.2 reacted with several forms of boar acrosin (55, 53, 45, and 38 kDa). All monoclonal antibodies labelled the acrosomes of undamaged spermatozoa in immunofluorescence test. This test should be convenient as an immunological test of the sperm quality.
References provided by Crossref.org
Fluorescent analysis of boar sperm capacitation process in vitro
Compartmentalization of the proteasome-interacting proteins during sperm capacitation
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Differential transferrin expression in placentae from normal and abnormal pregnancies: a pilot study
Genetic analysis of X-linked hybrid sterility in the house mouse
