Increased activity of c-Src and Csk in fibroblasts transformed by v-src oncogene
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11785970
DOI
10.1006/bbrc.2001.6247
PII: S0006291X01962473
Knihovny.cz E-resources
- MeSH
- Enzyme Activation drug effects MeSH
- Androstadienes pharmacology MeSH
- Cell Line MeSH
- CSK Tyrosine-Protein Kinase MeSH
- Fibroblasts cytology drug effects metabolism MeSH
- Enzyme Inhibitors pharmacology MeSH
- Cricetinae MeSH
- Blood Proteins pharmacology MeSH
- Oncogene Protein pp60(v-src) genetics metabolism MeSH
- Antibiotics, Antineoplastic pharmacology MeSH
- Proto-Oncogene Proteins pp60(c-src) metabolism MeSH
- Signal Transduction physiology MeSH
- Sirolimus pharmacology MeSH
- src-Family Kinases MeSH
- Protein-Tyrosine Kinases metabolism MeSH
- Cell Transformation, Viral physiology MeSH
- Avian Sarcoma Viruses genetics MeSH
- Wortmannin MeSH
- Animals MeSH
- Check Tag
- Cricetinae MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Androstadienes MeSH
- CSK Tyrosine-Protein Kinase MeSH
- Enzyme Inhibitors MeSH
- Blood Proteins MeSH
- Oncogene Protein pp60(v-src) MeSH
- Antibiotics, Antineoplastic MeSH
- Proto-Oncogene Proteins pp60(c-src) MeSH
- Sirolimus MeSH
- src-Family Kinases MeSH
- Protein-Tyrosine Kinases MeSH
- Wortmannin MeSH
When c-Src and v-Src were immunoprecipitated together from hamster fibroblasts transformed by Rous sarcoma virus containing v-src oncogene, the total Src activity was almost threefold higher compared to c-Src activity in the control cells. The activity of v-Src immunoprecipitated separately, however, accounting for only 40% of the total Src activity, indicating that c-Src is activated upon transformation. An increased activity of Csk was also found in RSV-transformed cells. It decreased upon serum stimulation in parallel with an increase in Src kinase activity. In nontransformed cells, serum stimulation induced an enhanced Csk activity, but no changes in c-Src activity were observed. This may suggest that Csk may have more functions in hamster fibroblasts, in addition to its inhibitory effect on c-Src.
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