Conserved guanine-guanine stacking in tetraplex and duplex DNA
Language English Country Germany Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
11820398
DOI
10.1007/s002490100174
Knihovny.cz E-resources
- MeSH
- Circular Dichroism MeSH
- DNA chemistry MeSH
- Electrophoresis, Polyacrylamide Gel MeSH
- Transcription, Genetic MeSH
- Guanine chemistry MeSH
- Nucleic Acid Conformation MeSH
- Protein Conformation MeSH
- Nucleic Acids chemistry MeSH
- Polytetrafluoroethylene pharmacology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA MeSH
- Guanine MeSH
- Nucleic Acids MeSH
- Polytetrafluoroethylene MeSH
Using a series of suitably chosen oligonucleotides, we demonstrate that the DNA duplex of d(CCCCGGGG) provides an almost identical CD spectrum as the parallel-stranded tetraplex of d(GGGG). The CD spectra are very sensitive to base stacking in DNA so that the above observation indicates that guanine-guanine stacking is essentially the same within the duplex of d(CCCCGGGG) and the tetraplex of d(GGGG). A very similar CD spectrum is also provided by the A-form of d(CCCCGGGG) induced by trifluoroethanol. These results reveal that guanine-guanine stacking is a structural invariant conserved in various nucleic acid conformers. The structural invariance is likely to cohere with evolution of the genetic molecules and be important for fundamental functions, e.g. initiation of transcription.
References provided by Crossref.org
Circular dichroism and conformational polymorphism of DNA
