Use of yeast two-hybrid system for detection of Bacillus subtilis FtsZ protein partners
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11830939
DOI
10.1007/bf02815616
Knihovny.cz E-zdroje
- MeSH
- Bacillus subtilis genetika metabolismus MeSH
- bakteriální proteiny genetika metabolismus MeSH
- beta-galaktosidasa metabolismus MeSH
- cytoskeletální proteiny * MeSH
- genomová knihovna MeSH
- plazmidy genetika MeSH
- techniky dvojhybridového systému * MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- beta-galaktosidasa MeSH
- cytoskeletální proteiny * MeSH
- FtsZ protein, Bacteria MeSH Prohlížeč
Yeast two-hybrid system was modified to allow easy detection of prokaryotic protein-protein interactions. Three plasmids (pGBR1, pGBR2, pGBR3) with the ClaI restriction site shifted in the three possible reading frames in fusion with GAL4 activating domain were constructed. The modified plasmids were used for identification of protein partners of FtsZ from Bacillus subtilis. Among partners of FtsZ the FtsA protein and a globular part of the SpoIIE protein were identified. The protein interactions were quantified by measurements of beta-galactosidase activity in yeast cells using 4-methylumbelliferyl beta-D-galactopyranoside as fluorogenic substrate.
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Genes Dev. 1991 Mar;5(3):447-55 PubMed
Science. 1995 Oct 27;270(5236):637-40 PubMed
Folia Microbiol (Praha). 1998;43(4):339-52 PubMed
Nature. 1989 Jul 20;340(6230):245-6 PubMed
J Bacteriol. 1998 Mar;180(5):1256-60 PubMed
J Bacteriol. 1997 Sep;179(17):5551-9 PubMed
EMBO J. 2000 Apr 3;19(7):1467-75 PubMed
Mol Microbiol. 1996 Mar;19(5):1047-60 PubMed
Mol Microbiol. 1999 Mar;31(5):1407-15 PubMed
Mol Microbiol. 1997 Sep;25(5):839-46 PubMed
J Bacteriol. 1996 Dec;178(24):7167-72 PubMed
Cell. 1985 Dec;43(3 Pt 2):729-36 PubMed
