Chromosome condensation in pig oocytes: lack of a requirement for either cdc2 kinase or MAP kinase activity
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
Grant support
R03-TW-05530-01
FIC NIH HHS - United States
R37 HD 22681
NICHD NIH HHS - United States
PubMed
12211068
DOI
10.1002/mrd.10176
Knihovny.cz E-resources
- MeSH
- Enzyme Activation drug effects MeSH
- Chromosomes physiology MeSH
- Cyclin-Dependent Kinases antagonists & inhibitors MeSH
- 4-Butyrolactone analogs & derivatives pharmacology MeSH
- Histones metabolism MeSH
- Enzyme Inhibitors pharmacology MeSH
- Okadaic Acid pharmacology MeSH
- Mitogen-Activated Protein Kinase 1 physiology MeSH
- Mitogen-Activated Protein Kinase 3 MeSH
- Mitogen-Activated Protein Kinases physiology MeSH
- Oocytes enzymology ultrastructure MeSH
- Swine genetics MeSH
- Phosphoprotein Phosphatases antagonists & inhibitors MeSH
- CDC2 Protein Kinase physiology MeSH
- Egg Proteins antagonists & inhibitors physiology MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, P.H.S. MeSH
- Names of Substances
- butyrolactone I MeSH Browser
- Cyclin-Dependent Kinases MeSH
- 4-Butyrolactone MeSH
- Histones MeSH
- Enzyme Inhibitors MeSH
- Okadaic Acid MeSH
- Mitogen-Activated Protein Kinase 1 MeSH
- Mitogen-Activated Protein Kinase 3 MeSH
- Mitogen-Activated Protein Kinases MeSH
- Phosphoprotein Phosphatases MeSH
- CDC2 Protein Kinase MeSH
- Egg Proteins MeSH
In this study, butyrolactone I (BL I), a potent and specific inhibitor of cyclin-dependent kinases (cdk), is shown to inhibit germinal vesicle breakdown (GVBD) in pig oocytes. Oocytes treated with 100 microM BL I were arrested in the germinal vesicle (GV)-stage and displayed low activity of cdc2 kinase and MAP kinase. Nevertheless, chromosome condensation occurred and highly condensed bivalents were seen within an intact GV after a 24-hr culture in the presence of BL I. The inhibitory effect of BL I on MAP kinase activation during culture was likely mediated through a cdk-dependent pathway, since MAP kinase activity present in extracts derived from metaphase II eggs was not inhibited by BL I. The block of GVBD could be released by treating oocytes with okadaic acid (OA), an inhibitor of type 1 and 2A phosphatases; 82% of the oocytes treated with the combination of OA/BL I underwent GVBD, and MAP kinase became activated, while cdc2 kinase remained inhibited. These results suggest that both chromosome condensation and GVBD could occur without activation of cdc2 kinase, whereas an increase in MAP kinase activity may be a requisite for GVBD in pig oocytes in conditions when cdc2 kinase activation is blocked by BL I.
References provided by Crossref.org
Importance of ERK1/2 in Regulation of Protein Translation during Oocyte Meiosis
G2/M transition of pig oocytes: How do oocytes initiate maturation?
