CC and C chemokine expression in pulmonary sarcoidosis
Language English Country England, Great Britain Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Bronchoalveolar Lavage Fluid chemistry cytology MeSH
- Chemokine CCL2 genetics metabolism MeSH
- Chemokine CCL3 MeSH
- Chemokine CCL4 MeSH
- Chemokine CCL5 genetics metabolism MeSH
- Chemokines, C genetics metabolism MeSH
- Chemokines, CC genetics metabolism MeSH
- Adult MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Gene Expression MeSH
- Humans MeSH
- Macrophage Inflammatory Proteins genetics metabolism MeSH
- RNA, Messenger analysis MeSH
- Sarcoidosis, Pulmonary metabolism MeSH
- Reverse Transcriptase Polymerase Chain Reaction MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chemokine CCL2 MeSH
- Chemokine CCL3 MeSH
- Chemokine CCL4 MeSH
- Chemokine CCL5 MeSH
- Chemokines, C MeSH
- Chemokines, CC MeSH
- Macrophage Inflammatory Proteins MeSH
- RNA, Messenger MeSH
- XCL1 protein, human MeSH Browser
The chemokines RANTES (regulated on activation, T-cell expressed and secreted; CC chemokine ligand (CCL)-5) and monocyte inflammatory protein (MIP)-1alpha (CCL-3) have been implicated in the development of alveolitis in pulmonary sarcoidosis. The novel C chemokine single cysteine motif (SCM)-1alpha (XCL-1) and the CC chemokine monocyte chemoattractant protein (MCP)-1 (CCL-2) are also mononuclear-cell attractants and represent alternative candidate mediators of alveolitis. Therefore, the expression of MCP-1 and SCM-1alpha was investigated together with the expression of RANTES and MIP-1alpha in bronchoalveolar lavage fluid (BALF) from control subjects and patients with sarcoidosis. The relationship between chemokine expression and sarcoidosis clinical course was also explored. Messenger ribonucleic acid (mRNA) expression for all four chemokines was determined by semiquantitative reverse transcriptase-polymerase chain reaction of RNA extracted from unseparated bronchoalveolar cells (17 patients, 12 controls). RANTES, MIP-1alpha and MCP-1 proteins were measured by enzyme-linked immunosorbent assay of unconcentrated BALF (60 patients, 17 controls). MCP-1, and namely RANTES and SCM-1alpha mRNA expression was upregulated in sarcoidosis, particularly in patients with more advanced disease. RANTES, and namely MCP-1 concentrations were elevated in BALF samples obtained from patients; MCP-1 levels were most increased in patients with chest radiographic stage 2 disease and also in patients with persistent and recurrent disease. In conclusion, chemokines monocyte chemotactive protein-1 and single cysteine motif-1alpha are, in addition to RANTES, associated with the development of alveolitis in sarcoidosis and their expression parallels the disease course.
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