Positive findings of Borrelia burgdorferi in Culex (Culex) pipiens pipiens larvae in the surrounding of Brno city determined by the PCR method
Language English Country Poland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12498597
Knihovny.cz E-resources
- MeSH
- Borrelia burgdorferi genetics isolation & purification MeSH
- Culex microbiology MeSH
- DNA, Bacterial genetics MeSH
- Electrophoresis, Agar Gel MeSH
- Insect Vectors microbiology MeSH
- Larva MeSH
- Lyme Disease epidemiology microbiology MeSH
- Polymerase Chain Reaction MeSH
- Seasons MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- DNA, Bacterial MeSH
After first finding Borrelia in the midgut of imago mosquitoes, we concentrated on the presence of Borrelia in mosquito development stages--larvae of the third or fourth instar. In the summer season in the years of 2000-2001 a total of 439 Culex (Culex) pipiens pipiens larvae were collected from a barrel of rainwater in the Obrany holiday area of Brno city (East Moravia, Czech Republic). The larvae midgut was observed under dark-field microscopy. Ten DFM positive samples (2.28%) were further analysed using the single-tube nested PCR method for the presence of flagellum DNA sequence specific for Borrelia burgdorferi sensu lato, of which 5 were positive. Borrelian positivity of Culex (C.) pipiens pipiens larvae was 1.14%. One spirochete isolated strain in BSK-H medium was obtained. PCR detection for borrelian DNA of the isolated strain was negative. From these results we can conclude that a low percentage Borreliae can be also found in mosquito larvae and are likely to survive into imago stage.
