Polymerase Chain Reaction [polymerázová řetězová reakce]
- Terms
-
anchored PCR
dvoustupňová polymerázová řetězová reakce
inverzní PCR
inverzní polymerázová řetězová reakce
nested PCR
PCR
-
Anchored PCR
Anchored Polymerase Chain Reaction
Inverse PCR
Inverse Polymerase Chain Reaction
Nested PCR
Nested Polymerase Chain Reaction
PCR
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
- DUI
- D016133 MeSH Browser
- CUI
- M0024634
- Previous indexing
- Gene Amplification (1988-1989)
- History note
- 91(90); was see under GENE AMPLIFICATION 1990
- Public note
- 91; was see under GENE AMPLIFICATION 1990
Allowable subheadings
- CL
- classification 7
- EC
- economics 6
- ES
- ethics
- HI
- history 10
- IS
- instrumentation 42
- MT
- methods 814
- ST
- standards 26
- SN
- statistics & numerical data 20
- TD
- trends 19
- VE
- veterinary 67