In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

DUI

D016133 MeSH Prohlížeč

CUI

M0024634

Předchozí užití

Gene Amplification (1988-1989)

Historická pozn.

91(90); was see under GENE AMPLIFICATION 1990

Veřejná pozn.

91; was see under GENE AMPLIFICATION 1990