Isolation and identification of Streptomyces sp. and assay of its exocellular water-soluble blue pigments
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12503393
DOI
10.1007/bf02818787
Knihovny.cz E-resources
- MeSH
- Pigments, Biological chemistry metabolism toxicity MeSH
- Cell Wall chemistry MeSH
- Genes, rRNA MeSH
- Molecular Sequence Data MeSH
- Mice MeSH
- Soil Microbiology MeSH
- DNA, Ribosomal analysis MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Solubility MeSH
- Sequence Analysis, DNA MeSH
- Streptomyces classification growth & development isolation & purification ultrastructure MeSH
- Bacterial Typing Techniques MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Pigments, Biological MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
A bacterial strain producing a great amount of blue pigment during submerse fermentation was isolated and identified. Based on morphological characteristics, cell-wall chemotype and sequence of 16S rRNA gene, the strain should belong to the genus Streptomyces; it had 99.4% homology of 16S rRNA gene sequence with that of Streptomyces indigocolor. The pigment production by the strain was affected by carbon and nitrogen sources. The main components of the pigment mixture (detected by HPLC and TLC) were tentatively classified as actinorhodin-related compounds. The pigment was relatively stable against light and higher temperature but was sensitive to low pH. The preliminary acute-toxicity determination showed that the pigment was nontoxic (LD50 > 15 mg/g).
See more in PubMed
J Bacteriol. 1996 Apr;178(8):2238-44 PubMed
Hum Exp Toxicol. 1999 Mar;18(3):162-7 PubMed
Chem Rev. 1997 Nov 10;97(7):2465-2498 PubMed
Mol Biol Evol. 1987 Jul;4(4):406-25 PubMed
Folia Microbiol (Praha). 2000;45(5):407-13 PubMed
Trends Biochem Sci. 1998 Oct;23(10):403-5 PubMed
Folia Microbiol (Praha). 2000;45(5):397-406 PubMed
Arch Toxicol Suppl. 1998;20:407-18 PubMed
Evolution. 1985 Jul;39(4):783-791 PubMed
Microbiology (Reading). 2000 Mar;146 ( Pt 3):611-619 PubMed
Science. 1991 May 3;252(5006):675-9 PubMed
J Gen Microbiol. 1979 Sep;114(1):35-43 PubMed
Appl Microbiol. 1972 Feb;23(2):402-6 PubMed
Nucleic Acids Res. 1997 Dec 15;25(24):4876-82 PubMed
GENBANK
AF275257
