Isolation and identification of Streptomyces sp. and assay of its exocellular water-soluble blue pigments
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12503393
DOI
10.1007/bf02818787
Knihovny.cz E-zdroje
- MeSH
- biologické pigmenty chemie metabolismus toxicita MeSH
- buněčná stěna chemie MeSH
- geny rRNA MeSH
- molekulární sekvence - údaje MeSH
- myši MeSH
- půdní mikrobiologie MeSH
- ribozomální DNA analýza MeSH
- RNA ribozomální 16S genetika MeSH
- rozpustnost MeSH
- sekvenční analýza DNA MeSH
- Streptomyces klasifikace růst a vývoj izolace a purifikace ultrastruktura MeSH
- techniky typizace bakterií MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- biologické pigmenty MeSH
- ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
A bacterial strain producing a great amount of blue pigment during submerse fermentation was isolated and identified. Based on morphological characteristics, cell-wall chemotype and sequence of 16S rRNA gene, the strain should belong to the genus Streptomyces; it had 99.4% homology of 16S rRNA gene sequence with that of Streptomyces indigocolor. The pigment production by the strain was affected by carbon and nitrogen sources. The main components of the pigment mixture (detected by HPLC and TLC) were tentatively classified as actinorhodin-related compounds. The pigment was relatively stable against light and higher temperature but was sensitive to low pH. The preliminary acute-toxicity determination showed that the pigment was nontoxic (LD50 > 15 mg/g).
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GENBANK
AF275257