Response regulator ChiR regulates expression of chitinase gene, chiC, in Streptomyces coelicolor
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12503394
DOI
10.1007/bf02818788
Knihovny.cz E-resources
- MeSH
- Bacterial Proteins genetics metabolism MeSH
- Chitinases genetics metabolism MeSH
- Single-Strand Specific DNA and RNA Endonucleases MeSH
- Escherichia coli enzymology genetics MeSH
- Transcription, Genetic * MeSH
- Molecular Sequence Data MeSH
- Promoter Regions, Genetic MeSH
- Gene Expression Regulation, Bacterial * MeSH
- Restriction Mapping MeSH
- Amino Acid Sequence MeSH
- Base Sequence MeSH
- Streptomyces enzymology genetics MeSH
- Transcription Factors * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Bacterial Proteins MeSH
- ChiR protein, Streptomyces MeSH Browser
- chitinase C-1 MeSH Browser
- Chitinases MeSH
- Single-Strand Specific DNA and RNA Endonucleases MeSH
- Transcription Factors * MeSH
Transcription from the chiC promoter, directing expression of the chitinase gene, chiC, in Streptomyces coelicolor, was analyzed using xylE reporter gene and high-resolution S1-nuclease mapping. The transcription from the chiC promoter was induced by chitin, and this induction was dramatically reduced in the S. coelicolor chiR-disrupted strain. This indicated a dependence of chiC expression upon the chiR gene encoding a response regulator protein. To investigate this relationship, the S. coelicolor ChiR was overproduced using Escherichia coli T7 RNA polymerase expression system. However, gel mobility shift-assay with such a purified ChiR showed no binding in the chiC promoter region, which indicates a lack of specific phosphorylation of E. coli overproduced ChiR that is necessary for DNA-binding activity of response regulators.
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