Recent news related to substrates and inhibitors of plant amine oxidases
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12686157
DOI
10.1016/s1570-9639(03)00094-3
PII: S1570963903000943
Knihovny.cz E-resources
- MeSH
- Amine Oxidase (Copper-Containing) antagonists & inhibitors metabolism MeSH
- Pisum sativum enzymology MeSH
- Enzyme Inhibitors pharmacology MeSH
- Zea mays enzymology MeSH
- Plant Proteins antagonists & inhibitors metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amine Oxidase (Copper-Containing) MeSH
- Enzyme Inhibitors MeSH
- Plant Proteins MeSH
Reactions of pea diamine oxidase (PSAO) and maize polyamine oxidase (MPAO) with 1,4-bis(3-aminopropyl)-piperazine (BAPP), diethylenetriamine (DETA), dipropylenetriamine (DPTA), dehydrospermine (DHSP) and 3-oxapentane-1,5-diamine (OPD) were studied and compared. These reactions were characterised by kinetic measurements (kinetic constants, stoichiometry) and by measurements of absorption spectra (reaction mechanisms). In the case of oxidised polyamine compounds, the corresponding reaction products were determined using analytical methods (coloured trapping reactions, mass spectrometry, IR spectroscopy, thin layer chromatography). Some of the compounds were found to be substrates of PSAO and relatively potent inhibitors of MPAO (and vice versa) all at once. The others showed the same effect on both enzymes. This may have an importance for designing of experiments in physiological studies in plants.
References provided by Crossref.org
Inhibition of diamine oxidases and polyamine oxidases by diamine-based compounds
