Viability of basidiomycete strains after cryopreservation: comparison of two different freezing protocols
Language English Country United States Media print
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12800506
DOI
10.1007/bf02930959
Knihovny.cz E-resources
- MeSH
- Basidiomycota classification enzymology growth & development physiology MeSH
- Cryopreservation methods MeSH
- Culture Media MeSH
- Laccase MeSH
- Mycology methods MeSH
- Oxidoreductases metabolism MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Culture Media MeSH
- Laccase MeSH
- Oxidoreductases MeSH
The viability of 250 basidiomycete strains was determined after a 2-d and then after a 2-year storage under liquid nitrogen using two different freezing protocols. Using an original agar plug protocol (OP), 162 strains (65%) of the 250 strains survived a 2-d storage and 158 strains (63%) survived a 2-year storage in liquid nitrogen. Using a straw protocol (CP), 246 strains (98%) of the 250 strains survived a 2-d storage and 243 strains (97%) a 2-year storage in liquid nitrogen. In addition, other 106 strains were newly estimated using the CP protocol; 104 (98%) of them survived successfully a 2-d storage and 101 (95%) of them survived a 2-year storage in liquid nitrogen. The results indicate that the protocol used for cryopreservation can significantly influence strain survival. Markedly better results were obtained using the CP protocol.
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