Viability of basidiomycete strains after cryopreservation: comparison of two different freezing protocols
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
12800506
DOI
10.1007/bf02930959
Knihovny.cz E-zdroje
- MeSH
- Basidiomycota klasifikace enzymologie růst a vývoj fyziologie MeSH
- kryoprezervace metody MeSH
- kultivační média MeSH
- lakasa MeSH
- mykologie metody MeSH
- oxidoreduktasy metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- kultivační média MeSH
- lakasa MeSH
- oxidoreduktasy MeSH
The viability of 250 basidiomycete strains was determined after a 2-d and then after a 2-year storage under liquid nitrogen using two different freezing protocols. Using an original agar plug protocol (OP), 162 strains (65%) of the 250 strains survived a 2-d storage and 158 strains (63%) survived a 2-year storage in liquid nitrogen. Using a straw protocol (CP), 246 strains (98%) of the 250 strains survived a 2-d storage and 243 strains (97%) a 2-year storage in liquid nitrogen. In addition, other 106 strains were newly estimated using the CP protocol; 104 (98%) of them survived successfully a 2-d storage and 101 (95%) of them survived a 2-year storage in liquid nitrogen. The results indicate that the protocol used for cryopreservation can significantly influence strain survival. Markedly better results were obtained using the CP protocol.
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