Imunofluorescencná identifikácia limbálnych kmenových buniek v kultúre donorskej rohovky
[Immunofluorescence identification of limbal stem cells in a culture of donor corneas]
Language Slovak Country Czech Republic Media print
Document type English Abstract, Journal Article
PubMed
12898719
- MeSH
- Fluorescent Antibody Technique * MeSH
- Phosphopyruvate Hydratase analysis immunology MeSH
- Stem Cells chemistry cytology MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Limbus Corneae cytology MeSH
- Antibodies, Monoclonal MeSH
- Corneal Transplantation * MeSH
- Check Tag
- Humans MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
- Names of Substances
- Phosphopyruvate Hydratase MeSH
- Antibodies, Monoclonal MeSH
The purpose of this study was to identify limbal stem cells in the culture of human donor cornea. After standard cultivation procedure we used monoclonal antibody against alpha-enolase for identification of stem cells. This antibody serves as a probe for the identification of biochemical marker of stem cells. Using immunofluorescent microscopy we identified in specimens alpha-enolase positive cells which in vitro but not in situ showed also some morphologic differences from other cellular elements of the culture.
