Imunofluorescencná identifikácia limbálnych kmenových buniek v kultúre donorskej rohovky
[Immunofluorescence identification of limbal stem cells in a culture of donor corneas]

. 2003 Jun ; 59 (3) : 171-5.

Jazyk slovenština Země Česko Médium print

Typ dokumentu anglický abstrakt, časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/pmid12898719
Odkazy

PubMed 12898719

The purpose of this study was to identify limbal stem cells in the culture of human donor cornea. After standard cultivation procedure we used monoclonal antibody against alpha-enolase for identification of stem cells. This antibody serves as a probe for the identification of biochemical marker of stem cells. Using immunofluorescent microscopy we identified in specimens alpha-enolase positive cells which in vitro but not in situ showed also some morphologic differences from other cellular elements of the culture.

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