Square wave and elimination voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články, práce podpořená grantem, validační studie
PubMed
15110244
DOI
10.1016/j.bioelechem.2003.10.012
PII: S1567539404000076
Knihovny.cz E-zdroje
- MeSH
- chromozomy chemie MeSH
- DNA analýza chemie MeSH
- elektrochemie metody MeSH
- komplexní směsi analýza chemie MeSH
- oligonukleotidy analýza chemie MeSH
- senzitivita a specificita MeSH
- zidovudin analýza chemie MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- validační studie MeSH
- Názvy látek
- calf thymus DNA MeSH Prohlížeč
- DNA MeSH
- komplexní směsi MeSH
- oligonukleotidy MeSH
- zidovudin MeSH
Azidothymidine (AZT, 3'-azido-3'-deoxythymidine, Zidovudine, Retrovir) is an approved and widely used antiretroviral drug for the treatment of human immunodeficiency virus (HIV) infection. Dynamic electrochemical methods have been employed for the fast and inexpensive determination of this drug in natural samples. The electrochemical signal of AZT, resulting from the reduction of azido group, was studied by square wave voltammetry (SWV), linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS) using a hanging mercury drop electrode (HMDE). This paper explores the possibility of determining AZT in the presence of native (dsDNA) or denatured calf thymus DNA (ssDNA), and/or some synthetic oligodeoxynucleotides (ODNs). The detection limit of AZT in the absence and in the presence of ssDNA (10 microg/ml) is 1 and 250 nM, respectively. It was found that the signal of AZT is not substantially affected by the presence of DNA. We can therefore assume that the electrons are transferred through the adsorption layer of nucleic acids. By using the elimination procedure, both irreversible reduction signals of AZT and DNA are augmented. Moreover, the elimination signal in the peak-counterpeak form may indicate the adsorption of the analytes on the electrode surface preceding an electron transfer.
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