Short-lived fluorescence component of DPH reports on lipid--water interface of biological membranes
Language English Country Ireland Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15172830
DOI
10.1016/j.chemphyslip.2004.02.005
PII: S0009308404000271
Knihovny.cz E-resources
- MeSH
- 1,2-Dipalmitoylphosphatidylcholine pharmacology MeSH
- Benzyl Alcohol pharmacology MeSH
- Time Factors MeSH
- Diphenylhexatriene analysis chemistry MeSH
- Ethanol pharmacology MeSH
- Fluorescence MeSH
- Fluorescence Polarization MeSH
- Lipids chemistry MeSH
- Liposomes chemistry MeSH
- Temperature MeSH
- Water chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1,2-Dipalmitoylphosphatidylcholine MeSH
- Benzyl Alcohol MeSH
- Diphenylhexatriene MeSH
- Ethanol MeSH
- Lipids MeSH
- Liposomes MeSH
- Water MeSH
Fluorescence measurements of 1,6-diphenyl-1,3,5-hexatriene (DPH) in large unilamellar phospholipid vesicles were performed to characterize the influence of the membrane physical properties on the short-lived lifetime component of the fluorescence decay. We have found that the short-lived component of DPH significantly shortens when the membrane undergoes a temperature-induced phase transition as it is known for the long-lived component of DPH. We induced membrane phase transitions also by alcohols, which are reported to be distributed different way in the membrane--ethanol close to the membrane-water interface and benzyl alcohol in the membrane core. A different effect of the respective alcohol on the short and long decay component was observed. Both the time-resolved fluorescence spectra of DPH taken during lipid vesicle staining and the lifetime dependences caused by changes of temperature and/or induced by the alcohols show that the short-lived fluorescence originates from the population of dye molecules distributed at the membrane-water interface.
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