Bacterial diversity in Malan ice core from the Tibetan Plateau
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15259767
DOI
10.1007/bf02931042
Knihovny.cz E-zdroje
- MeSH
- Bacteria klasifikace genetika izolace a purifikace MeSH
- geologické sedimenty mikrobiologie MeSH
- led * MeSH
- mikrobiologie vody * MeSH
- ribozomální DNA analýza MeSH
- RNA ribozomální 16S analýza genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Tibet MeSH
- Názvy látek
- led * MeSH
- ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
Three ice core samples were collected from the Malan ice core drilled from the Tibetan Plateau, and three 16S rDNA clone libraries by direct amplification from the ice-melted water were established. Ninety-four clones containing bacterial 16S rDNA inserts were selected. According to restriction fragment-length polymorphism analysis, 11 clones were unique in the library from which they were obtained and used for partial sequence and phylogenetic analysis, and compared with 8 reported sequences from the same ice core at depth 70 m. Differences among the samples were apparent in clone libraries. The phylotypes were dominated by the Proteobacteria group, Acinetobacter sp. and Cytophaga-Flavobacterium-Bacteroides (CFB) group. They accounted for 92.5% (Proteobacteria), 100% (Acinetobacter sp.), 34.4% (CFB) and 100% (beta-Proteobacteria) in the clone libraries from the samples at ice depths 35, 64, 70, and 82 m, respectively. The Acinetobacter sp. was only found in the deposition at ice depth 82 m and closely clustered with gamma-Proteobateria. Two members (Malan A-21 and 101) of alpha-Proteobacteria from the sample of 35 m and two (Malan B-26 and 48) of beta-Proteobacteria of 64 m were loosely clustered (< 95% similarity) with known bacteria, represented new genera in ice bacteria.
Zobrazit více v PubMed
Nucleic Acids Res. 1996 Jan 1;24(1):82-5 PubMed
Microb Ecol. 1997 May-Jun;33(3):169-79 PubMed
Folia Microbiol (Praha). 2002;47(3):241-5 PubMed
Polar Biol. 1988;9:37-44 PubMed
Int J Syst Bacteriol. 1997 Apr;47(2):278-83 PubMed
Folia Microbiol (Praha). 2003;48(3):379-84 PubMed
Science. 1998 Jun 26;280(5372):2095-8 PubMed
Appl Environ Microbiol. 1997 Aug;63(8):3068-78 PubMed
Microbiology. 1997 Dec;143 ( Pt 12):3913-9 PubMed
Appl Environ Microbiol. 1995 Sep;61(9):3486-9 PubMed
J Mol Biol. 1990 Oct 5;215(3):403-10 PubMed
Int J Syst Bacteriol. 1998 Jan;48 Pt 1:223-35 PubMed
Syst Appl Microbiol. 1998 Jun;21(2):306-14 PubMed
Sedimentology. 1991;38:363-79 PubMed
Science. 1999 Dec 10;286(5447):2141-4 PubMed
